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1j75
From Proteopedia
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Crystal Structure of the DNA-Binding Domain Zalpha of DLM-1 Bound to Z-DNA
Overview
The first crystal structure of a protein, the Z alpha high affinity, binding domain of the RNA editing enzyme ADAR1, bound to left-handed Z-DNA, was recently described. The essential set of residues determined from this, structure to be critical for Z-DNA recognition was used to search the, database for other proteins with the potential for Z-DNA binding. We found, that the tumor-associated protein DLM-1 contains a domain with remarkable, sequence similarities to Z alpha(ADAR). Here we report the crystal, structure of this DLM-1 domain bound to left-handed Z-DNA at 1.85 A, resolution. Comparison of Z-DNA binding by DLM-1 and ADAR1 reveals a, common structure-specific recognition core within the binding domain., However, the domains differ in certain residues peripheral to the, protein-DNA interface. These structures reveal a general mechanism of, Z-DNA recognition, suggesting the existence of a family of winged-helix, proteins sharing a common Z-DNA binding motif.
About this Structure
1J75 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Structure of the DLM-1-Z-DNA complex reveals a conserved family of Z-DNA-binding proteins., Schwartz T, Behlke J, Lowenhaupt K, Heinemann U, Rich A, Nat Struct Biol. 2001 Sep;8(9):761-5. PMID:11524677
Page seeded by OCA on Tue Nov 20 17:58:54 2007
