1jke
From Proteopedia
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D-Tyr tRNATyr deacylase from Escherichia coli
Overview
Cell growth inhibition by several d-amino acids can be explained by an in, vivo production of d-aminoacyl-tRNA molecules. Escherichia coli and yeast, cells express an enzyme, d-Tyr-tRNA(Tyr) deacylase, capable of recycling, such d-aminoacyl-tRNA molecules into free tRNA and d-amino acid., Accordingly, upon inactivation of the genes of the above deacylases, the, toxicity of d-amino acids increases. Orthologs of the deacylase are found, in many cells. In this study, the crystallographic structure of dimeric E., coli d-Tyr-tRNA(Tyr) deacylase at 1.55 A resolution is reported. The, structure corresponds to a beta-barrel closed on one side by a beta-sheet, lid. This barrel results from the assembly of the two subunits. Analysis, of the structure in relation with sequence homologies in the orthologous, family suggests the location of the active sites at the carboxy end of the, beta-strands. The solved structure markedly differs from those of all, other documented tRNA-dependent hydrolases.
About this Structure
1JKE is a Single protein structure of sequence from Escherichia coli with ZN as ligand. Full crystallographic information is available from OCA.
Reference
Structure of crystalline D-Tyr-tRNA(Tyr) deacylase. A representative of a new class of tRNA-dependent hydrolases., Ferri-Fioni ML, Schmitt E, Soutourina J, Plateau P, Mechulam Y, Blanquet S, J Biol Chem. 2001 Dec 14;276(50):47285-90. Epub 2001 Sep 21. PMID:11568181
Page seeded by OCA on Tue Nov 20 18:20:36 2007
