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1jqt
From Proteopedia
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Fitting of L11 protein in the low resolution cryo-EM map of E.coli 70S ribosome
Overview
L11 protein is located at the base of the L7/L12 stalk of the 50 S subunit, of the Escherichia coli ribosome. Because of the flexible nature of the, region, recent X-ray crystallographic studies of the 50 S subunit failed, to locate the N-terminal domain of the protein. We have determined the, position of the complete L11 protein by comparing a three-dimensional, cryo-EM reconstruction of the 70 S ribosome, isolated from a mutant, lacking ribosomal protein L11, with the three-dimensional map of the, wild-type ribosome. Fitting of the X-ray coordinates of L11-23 S RNA, complex and EF-G into the cryo-EM maps combined with molecular modeling, reveals that, following EF-G-dependent GTP hydrolysis, domain V of EF-G, intrudes into the cleft between the 23 S ribosomal RNA and the N-terminal, domain of L11 (where the antibiotic thiostrepton binds), causing the, N-terminal domain to move and thereby inducing the formation of the, arc-like connection with the G' domain of EF-G. The results provide a new, insight into the mechanism of EF-G-dependent translocation.
About this Structure
1JQT is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Localization of L11 protein on the ribosome and elucidation of its involvement in EF-G-dependent translocation., Agrawal RK, Linde J, Sengupta J, Nierhaus KH, Frank J, J Mol Biol. 2001 Aug 24;311(4):777-87. PMID:11518530
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