1jvt
From Proteopedia
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CRYSTAL STRUCTURE OF RIBONUCLEASE A (LIGAND-FREE FORM)
Overview
Despite the increasing number of successful determinations of complex, protein structures the understanding of their dynamics properties is still, rather limited. Using X-ray crystallography, we demonstrate that, ribonuclease A (RNase A) undergoes significant domain motions upon ligand, binding. In particular, when cytidine 2'-monophosphate binds to RNase A, the structure of the enzyme becomes more compact. Interestingly, our data, also show that these structural alterations are fully reversible in the, crystal state. These findings provide structural bases for the dynamic, behavior of RNase A in the binding of the substrate shown by Petsko and, coworkers (Rasmussen et al. Nature 1992;357:423-424). These subtle domain, motions may assume functional relevance for more complex system and may, play a significant role in the cooperativity of oligomeric enzymes.
About this Structure
1JVT is a Single protein structure of sequence from Bos taurus. Active as Pancreatic ribonuclease, with EC number 3.1.27.5 Full crystallographic information is available from OCA.
Reference
Reversible substrate-induced domain motions in ribonuclease A., Vitagliano L, Merlino A, Zagari A, Mazzarella L, Proteins. 2002 Jan 1;46(1):97-104. PMID:11746706
Page seeded by OCA on Tue Nov 20 18:37:04 2007
