1k0t

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NMR SOLUTION STRUCTURE OF UNBOUND, OXIDIZED PHOTOSYSTEM I SUBUNIT PSAC, CONTAINING [4FE-4S] CLUSTERS FA AND FB

Overview

This work presents the three-dimensional NMR solution structure of, recombinant, oxidized, unbound PsaC from Synechococcus sp. PCC 7002., Constraints are derived from homo- and heteronuclear one-, two- and, three-dimensional (1)H and (15)N NMR data. Significant differences are, outlined between the unbound PsaC structure presented here and the, available X-ray structure of bound PsaC as an integral part of the whole, cyanobacterial PS I complex. These differences mainly concern the, arrangement of the N- and C-termini with respect to the [4Fe-4S] core, domain. In the NMR solution structure of PsaC the C-terminal region, assumes a disordered helical conformation, and is clearly different from, the extended coil conformation, which is one of the structural elements, required to anchor PsaC to the PS I core heterodimer. In solution the, N-terminus of PsaC is in contact with the pre-C-terminal region but slides, in between the latter and the iron-sulfur core region of the protein., Together, these features result in a concerted movement of the N-terminus, and pre-C-terminal region away from the F(A) binding site, accompanied by, a bending of the N-terminus. In comparison, the same terminal regions are, positioned much closer to F(A) and take up an anti-parallel beta-sheet, arrangement in PsaC bound to PS I. The conformational changes between, bound and unbound PsaC correlate with the differences reported earlier for, the EPR spectra of reduced F(A) and F(B) in bound versus unbound PsaC. The, observed different structural features in solution are highly relevant for, unraveling the stepwise assembly process of the stromal PsaC, PsaD and, PsaE subunits to the PS I core heterodimer. Electronic supplementary, material to this paper can be obtained by using the Springer Link server, located at http://dx.doi.org/10.1007/s00775-001-0321-3.

About this Structure

1K0T is a Single protein structure of sequence from Synechococcus sp. with SF4 as ligand. Full crystallographic information is available from OCA.

Reference

Solution structure of the unbound, oxidized Photosystem I subunit PsaC, containing [4Fe-4S] clusters F(A) and F(B): a conformational change occurs upon binding to photosystem I., Antonkine ML, Liu G, Bentrop D, Bryant DA, Bertini I, Luchinat C, Golbeck JH, Stehlik D, J Biol Inorg Chem. 2002 Apr;7(4-5):461-72. Epub 2002 Jan 11. PMID:11941504

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