1k2w
From Proteopedia
|
Crystal structure of sorbitol dehydrogenase from R. sphaeroides
Overview
Recombinant sorbitol dehydrogenase (SDH) from Rhodobacter sphaeroides has, been crystallized in the absence of the cofactor NAD(H) and its structure, determined to 2.4 A resolution using molecular replacement (refined R and, R free factors of 18.8 and 23.8%, respectively). As expected from the, sequence and shown by the conserved fold, SDH can be assigned to the, short-chain dehydrogenase/reductase protein family. The cofactor NAD and, the substrate sorbitol have been modelled into the structure and the, active-site architecture, which displays the highly conserved catalytic, tetrad of Asn-Ser-Tyr-Lys residues, is discussed in relation to the enzyme, mechanism. This is the first structure of a bacterial SDH belonging to the, SDR family.
About this Structure
1K2W is a Single protein structure of sequence from Rhodobacter sphaeroides. Active as L-iditol 2-dehydrogenase, with EC number 1.1.1.14 Full crystallographic information is available from OCA.
Reference
Structure of zinc-independent sorbitol dehydrogenase from Rhodobacter sphaeroides at 2.4 A resolution., Philippsen A, Schirmer T, Stein MA, Giffhorn F, Stetefeld J, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):374-9. Epub 2005, Mar 24. PMID:15805591
Page seeded by OCA on Tue Nov 20 18:48:26 2007
