1kdg

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spinqualitylabelsall models 1kdg, resolution 1.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of the flavin domain of cellobiose dehydrogenase

Overview

Cellobiose dehydrogenase (CDH) participates in the degradation of, cellulose and lignin. The protein is an extracellular flavocytochrome with, a b-type cytochrome domain (CYT(cdh)) connected to a flavodehydrogenase, domain (DH(cdh)). DH(cdh) catalyses a two-electron oxidation at the, anomeric C1 position of cellobiose to yield cellobiono-1,5-lactone, and, the electrons are subsequently transferred from DH(cdh) to an acceptor, either directly or via CYT(cdh). Here, we describe the crystal structure, of Phanerochaete chrysosporium DH(cdh) determined at 1.5 A resolution., DH(cdh) belongs to the GMC family of oxidoreductases, which includes, glucose oxidase (GOX) and cholesterol oxidase (COX); however, the sequence, identity with members of the family is low. The overall fold of DH(cdh) is, p-hydroxybenzoate hydroxylase-like and is similar to, but also different, from, that of GOX and COX. It is partitioned into an FAD-binding subdomain, of alpha/beta type and a substrate-binding subdomain consisting of a, seven-stranded beta sheet and six helices. Docking of CYT(cdh) and DH(cdh), suggests that CYT(cdh) covers the active-site entrance in DH(cdh), and, that the resulting distance between the cofactors is within acceptable, limits for inter-domain electron transfer. Based on docking of the, substrate, cellobiose, in the active site of DH(cdh), we propose that the, enzyme discriminates against glucose by favouring interaction with the, non-reducing end of cellobiose.

About this Structure

1KDG is a Single protein structure of sequence from Phanerochaete chrysosporium with NAG, MAN, HG, 6FA, EMT and S as ligands. Full crystallographic information is available from OCA.

Reference

Crystal structure of the flavoprotein domain of the extracellular flavocytochrome cellobiose dehydrogenase., Hallberg BM, Henriksson G, Pettersson G, Divne C, J Mol Biol. 2002 Jan 18;315(3):421-34. PMID:11786022

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