3por
From Proteopedia
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PORIN CONFORMATION IN THE ABSENCE OF CALCIUM; REFINED STRUCTURE AT 2.5 ANGSTROMS RESOLUTION
Overview
The crystal structure of porin from Rhodobacter capsulatus in the absence, of divalent calcium ions has been refined to convergence at a resolution, of 2.5 A using the simulated annealing refinement method. The final model, consists of all 301 amino acid residues, 77 solvent molecules, one, tris(hydroxymethyl)-aminomethane molecule and one unknown ligand modeled, as n-octyltetraoxyethylene. A superposition with the previously described, model containing three calcium ions showed structural changes at the, segment 108-116 of the inner loop beta 5-beta 6, and at loops beta 8-beta, 9 and beta 11-beta 12 at the extracellular side of the porin molecule., Evidence is presented that the conformational changes depend on the, presence or absence of calcium ions. A possible influence on porin, function is discussed.
About this Structure
3POR is a Single protein structure of sequence from Rhodobacter capsulatus with C8E and TRS as ligands. Full crystallographic information is available from OCA.
Reference
Porin conformation in the absence of calcium. Refined structure at 2.5 A resolution., Weiss MS, Schulz GE, J Mol Biol. 1993 Jun 5;231(3):817-24. PMID:7685826
Page seeded by OCA on Tue Nov 20 19:56:27 2007
