1lgt
From Proteopedia
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CRYSTAL STRUCTURE OF 2,3-DIHYDROXYBIPHENYL 1,2-DIOXYGENASE (DHBD) COMPLEXED WITH 2'-Cl DIHYDROXYBIPHENYL (DHB)
Overview
The microbial degradation of polychlorinated biphenyls (PCBs) provides the, potential to destroy these widespread, toxic and persistent environmental, pollutants. For example, the four-step upper bph pathway transforms some, of the more than 100 different PCBs found in commercial mixtures and is, being engineered for more effective PCB degradation. In the critical third, step of this pathway, 2,3-dihydroxybiphenyl (DHB) 1,2-dioxygenase (DHBD;, EC 1.13.11.39) catalyzes aromatic ring cleavage. Here we demonstrate that, ortho-chlorinated PCB metabolites strongly inhibit DHBD, promote its, suicide inactivation and interfere with the degradation of other, compounds. For example, k(cat)(app) for 2',6'-diCl DHB was reduced by a, factor of approximately 7,000 relative to DHB, and it bound with, sufficient affinity to competitively inhibit DHB cleavage at nanomolar, concentrations. Crystal structures of two complexes of DHBD with, ortho-chlorinated metabolites at 1.7 A resolution reveal an explanation, for these phenomena, which have important implications for bioremediation, strategies.
About this Structure
1LGT is a Single protein structure of sequence from Burkholderia sp. with FE2 and BP3 as ligands. Active as Biphenyl-2,3-diol 1,2-dioxygenase, with EC number 1.13.11.39 Full crystallographic information is available from OCA.
Reference
Identification and analysis of a bottleneck in PCB biodegradation., Dai S, Vaillancourt FH, Maaroufi H, Drouin NM, Neau DB, Snieckus V, Bolin JT, Eltis LD, Nat Struct Biol. 2002 Dec;9(12):934-9. PMID:12415290
Page seeded by OCA on Tue Nov 20 20:33:14 2007
