1lrp

From Proteopedia

proteopedia linkproteopedia link

spinqualitylabelsall models 1lrp, resolution 3.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

COMPARISON OF THE STRUCTURES OF CRO AND LAMBDA REPRESSOR PROTEINS FROM BACTERIOPHAGE LAMBDA

Overview

The three-dimensional structures of cro repressor protein and of the, amino-terminal domain of lambda repressor protein, both from bacteriophage, lambda, are compared. The second and third alpha-helices, alpha 2 and, alpha 3, are shown to have essentially identical conformations in the two, proteins, confirming the significance of the amino acid sequence homology, previously noted between these and other DNA binding proteins in the, region corresponding to these helices. The correspondence between the, two-helical units in cro and lambda repressor protein is better than the, striking agreement noted previously between two-helical units in cro and, catabolite gene-activator protein. Parts of the first alpha-helices of, repressor and cro show a structural correspondence that suggests a revised, sequence homology between the two proteins in their extreme amino-terminal, regions. In particular, there is a short loop between the alpha 1 and, alpha 2 helices of lambda repressor that is missing from cro. This, structural difference may account for the observed differences found with, different cros and repressors in the pattern of phosphates whose, ethylation prevents the binding of these proteins to their specific, recognition sites. Although the two proteins have strikingly similar alpha, 2-alpha 3 helical units that are presumed to bind to DNA in an essentially, similar manner, stereochemical restrictions prevent the alpha 2-alpha 3, units of the respective proteins aligning on the DNA in exactly the same, way.

About this Structure

1LRP is a Single protein structure of sequence from Enterobacteria phage lambda. Full crystallographic information is available from OCA.

Reference

Comparison of the structures of cro and lambda repressor proteins from bacteriophage lambda., Ohlendorf DH, Anderson WF, Lewis M, Pabo CO, Matthews BW, J Mol Biol. 1983 Sep 25;169(3):757-69. PMID:6226802

Page seeded by OCA on Tue Nov 20 20:49:04 2007