1yfm
From Proteopedia
|
RECOMBINANT YEAST FUMARASE
Overview
Crystal structures for both native and recombinant forms of yeast fumarase, from Saccharomyces cerevisiae have been completed to moderate resolution, by two separate laboratories. The recombinant form was obtained by the, construction of an expression plasmid for Escherichia coli. Despite a high, level of amino acid sequence similarity, purification of the eukaryotic, enzyme from the wild-type prokaryotic enzyme was feasible. The crystal, structure of the native form, NY-fumarase, encompasses residues R22, through M484, while the recombinant form, RY-fumarase, consists of, residues S27 through L485. Both crystal structures lack the N-terminal, translocation segment. Each subunit of the homo-tetrameric protein has, three domains. The active site is formed by segments from each of three, ... [(full description)]
About this Structure
1YFM is a [Single protein] structure of sequence from [Saccharomyces cerevisiae]. Active as [[1]], with EC number [4.2.1.2]. Full crystallographic information is available from [OCA].
Reference
Crystal structures of native and recombinant yeast fumarase., Weaver T, Lees M, Zaitsev V, Zaitseva I, Duke E, Lindley P, McSweeny S, Svensson A, Keruchenko J, Keruchenko I, Gladilin K, Banaszak L, J Mol Biol. 1998 Jul 17;280(3):431-42. PMID:9665847
Page seeded by OCA on Mon Oct 29 20:24:48 2007
