1gt6
From Proteopedia
|
S146A MUTANT OF THERMOMYCES (HUMICOLA) LANUGINOSA LIPASE COMPLEX WITH OLEIC ACID
Overview
The binding of Thermomyces lanuginosa lipase and its mutants [TLL(S146A), TLL(W89L), TLL(W117F, W221H, W260H)] to the mixed micelles of, cis-parinaric acid/sodium taurodeoxycholate at pH 5.0 led to the quenching, of the intrinsic tryptophan fluorescence emission (300-380 nm) and to a, simultaneous increase in the cis-parinaric acid fluorescence emission, (380-500 nm). These findings were used to characterize the Thermomyces, lanuginosa lipase/cis-parinaric acid interactions occurring in the, presence of sodium taurodeoxycholate.The fluorescence resonance energy, transfer and Stern-Volmer quenching constant values obtained were, correlated with the accessibility of the tryptophan residues to the, cis-parinaric acid and with the lid opening ability of Thermomyces, lanuginosa lipase (and its ... [(full description)]
About this Structure
1GT6 is a [Single protein] structure of sequence from [Thermomyces lanuginosus] with OLA as [ligand]. Active as [[1]], with EC number [3.1.1.3]. Full crystallographic information is available from [OCA].
Reference
Binding of Thermomyces (Humicola) lanuginosa lipase to the mixed micelles of cis-parinaric acid/NaTDC., Yapoudjian S, Ivanova MG, Brzozowski AM, Patkar SA, Vind J, Svendsen A, Verger R, Eur J Biochem. 2002 Mar;269(6):1613-21. PMID:11895431
Page seeded by OCA on Mon Oct 29 20:25:43 2007
