1a54

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spinqualitylabelsall models 1a54, resolution 1.6Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

PHOSPHATE-BINDING PROTEIN MUTANT A197C LABELLED WITH A COUMARIN FLUOROPHORE AND BOUND TO DIHYDROGENPHOSPHATE ION

Overview

Crystal structures are presented for the A197C mutant of Escherichia coli, phosphate binding protein (PBP) and the same mutant labeled at Cys197 with, N-[2-(1-maleimidyl)ethyl]-7-(diethylamino)coumarin-3-carboxamide (MDCC)., Both proteins are complexed with inorganic phosphate. The latter molecule, MDCC-PBP, exhibits a large increase in fluorescence on binding inorganic, phosphate. The resulting high-fluorescence state of the coumarin and the, ability of this coumarin to monitor the conformational changes associated, with inorganic phosphate binding are interpreted in terms of the specific, interactions of MDCC with the protein. The structure helps to explain why, this particular label gives a high-fluorescence state on binding inorganic, phosphate, while several other related labels do ... [(full description)]

About this Structure

1A54 is a [Single protein] structure of sequence from [Escherichia coli] with 2HP and MDC as [ligands]. Full crystallographic information is available from [OCA].

Reference

Crystal structure of phosphate binding protein labeled with a coumarin fluorophore, a probe for inorganic phosphate., Hirshberg M, Henrick K, Haire LL, Vasisht N, Brune M, Corrie JE, Webb MR, Biochemistry. 1998 Jul 21;37(29):10381-5. PMID:9671506

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