1maa

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spinqualitylabelsall models 1maa, resolution 2.9Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

MOUSE ACETYLCHOLINESTERASE CATALYTIC DOMAIN, GLYCOSYLATED PROTEIN

Overview

The crystal structure of mouse acetylcholinesterase at 2.9-A resolution, reveals a tetrameric assembly of subunits with an antiparallel alignment, of two canonical homodimers assembled through four-helix bundles. In the, tetramer, a short Omega loop, composed of a cluster of hydrophobic, residues conserved in mammalian acetylcholinesterases along with flanking, alpha-helices, associates with the peripheral anionic site of the facing, subunit and sterically occludes the entrance of the gorge leading to the, active center. The inverse loop-peripheral site interaction occurs within, the second pair of subunits, but the peripheral sites on the two, loop-donor subunits remain freely accessible to the solvent. The position, and complementarity of the peripheral site-occluding loop mimic the, characteristics of the central loop of the peptidic inhibitor fasciculin, bound to mouse acetylcholinesterase. Tetrameric forms of cholinesterases, are widely distributed in nature and predominate in mammalian brain. This, structure reveals a likely mode of subunit arrangement and suggests that, the peripheral site, located near the rim of the gorge, is a site for, association of neighboring subunits or heterologous proteins with, interactive surface loops.

About this Structure

1MAA is a Single protein structure of sequence from Mus musculus with NAG, PO4, DME and GOL as ligands. Active as Acetylcholinesterase, with EC number 3.1.1.7 Full crystallographic information is available from OCA.

Reference

Crystal structure of mouse acetylcholinesterase. A peripheral site-occluding loop in a tetrameric assembly., Bourne Y, Taylor P, Bougis PE, Marchot P, J Biol Chem. 1999 Jan 29;274(5):2963-70. PMID:9915834

Page seeded by OCA on Tue Nov 20 21:16:15 2007