1up0

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spinqualitylabelsall models 1up0, resolution 1.75Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

STRUCTURE OF THE ENDOGLUCANASE CEL6 FROM MYCOBACTERIUM TUBERCULOSIS IN COMPLEX WITH CELLOBIOSE AT 1.75 ANGSTROM

Overview

The genomes of various Mycobacterium tuberculosis strains encode proteins, that do not appear to play a role in the growth or survival of the, bacterium in its mammalian host, including some implicated in plant cell, wall breakdown. Here we show that M. tuberculosis H37Rv does indeed, possess a functional cellulase. The x-ray crystal structure of this, enzyme, in ligand complex forms, from 1.9 to 1.1A resolution, reveals a, highly conserved substrate-binding cleft, which affords similar, and, unusual, distortion of the substrate at the catalytic center. The, endoglucanase activity, together with the existence of a putative, membrane-associated crystalline polysaccharide-binding protein, may, reflect the ancestral soil origin of the Mycobacterium or hint at a, previously unconsidered ... [(full description)]

About this Structure

1UP0 is a [Single protein] structure of sequence from [Mycobacterium tuberculosis] with SO4, ACT and 1PG as [ligands]. Full crystallographic information is available from [OCA].

Reference

Mycobacterium tuberculosis strains possess functional cellulases., Varrot A, Leydier S, Pell G, Macdonald JM, Stick RV, Henrissat B, Gilbert HJ, Davies GJ, J Biol Chem. 2005 May 27;280(21):20181-4. Epub 2005 Apr 11. PMID:15824123

Page seeded by OCA on Mon Oct 29 20:29:31 2007