1mse
From Proteopedia
|
SOLUTION STRUCTURE OF A SPECIFIC DNA COMPLEX OF THE MYB DNA-BINDING DOMAIN WITH COOPERATIVE RECOGNITION HELICES
Overview
The DNA-binding region of Myb consists of three imperfect tandem repeats, (R1, R2, and R3). We have determined the solution structure of a specific, DNA complex of the minimum DNA-binding domain (R2R3) by heteronuclear, multidimensional NMR. Both R2 and R3 contain three helices, and the third, helix in each is found to be a recognition helix. R2 and R3 are closely, packed in the major groove, so that the two recognition helices contact, each other directly to bind to the specific base sequence, AACNG, cooperatively; this is a significant arrangement of recognition helices., The three key base pairs in this sequence are specifically recognized by, Asn-183 (R3), Lys-182 (R3), and Lys-128 (R2). In contrast, R1 has no, specific interactions with DNA from our NMR study of the DNA complex of, the full DNA-binding domain (R1R2R3).
About this Structure
1MSE is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Solution structure of a specific DNA complex of the Myb DNA-binding domain with cooperative recognition helices., Ogata K, Morikawa S, Nakamura H, Sekikawa A, Inoue T, Kanai H, Sarai A, Ishii S, Nishimura Y, Cell. 1994 Nov 18;79(4):639-48. PMID:7954830
Page seeded by OCA on Tue Nov 20 21:40:03 2007
