1n5h
From Proteopedia
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Solution structure of the cathelin-like domain of protegrins (the R87-P88 and D118-P119 amide bonds are in the cis conformation)
Overview
In mammals, numerous precursors of antibacterial peptides with unrelated, sequences share a similar prosequence of 94-114 residues, termed the, cathelin-like domain. The cathelin-like domain of protegrin-3 (ProS) was, overexpressed in Escherichia coli and uniformly labeled with (15)N or, (15)N and (13)C, and its three-dimensional structure was determined by, heteronuclear NMR at pH 6.2. Under these conditions and due to the, cis-trans isomerization of the R(87)-P(88) and D(118)-P(119) amide bonds, the ProS structure was found to adopt four almost equally populated, conformations in slow exchange on the NMR chemical shift time scale. The, ProS structure consists of an N-terminal alpha-helix (Y(34)-N(48)) cradled, by a four-stranded antiparallel beta-sheet (beta1, N(53)-L(60); beta2, K(74)-P(86); beta3, V(104)-V(111); and beta4, I(122)-C(124)). The solution, structure of ProS, which is monomeric, allowed us to determine the, structure of the L1 and L2 loops, which are too mobile in the crystal, structure. The regions common to the solution and X-ray structures were, found to be very similar. Finally, since the overall fold of ProS is very, similar to that of cystatins despite a low degree of sequence identity, the ProS solution structure was compared to the solution and X-ray, structures of the chicken cystatin. This comparison revealed that the, structures of the L1 and L2 loops as well as that of the appending domain, are quite different in the two proteins. These differences are mainly due, to the high proline residue content (10%) which disorganizes the hydrogen, bond network of a part of the ProS beta-sheet in contrast to that of the, chicken cystatin structure.
About this Structure
1N5H is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.
Reference
NMR structure of the cathelin-like domain of the protegrin-3 precursor., Yang Y, Sanchez JF, Strub MP, Brutscher B, Aumelas A, Biochemistry. 2003 Apr 29;42(16):4669-80. PMID:12705830
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