1n7e

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spinqualitylabelsall models 1n7e, resolution 1.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of the sixth PDZ domain of GRIP1

Overview

PDZ domains bind to short segments within target proteins in a, sequence-specific fashion. Glutamate receptor-interacting protein, (GRIP)/ABP family proteins contain six to seven PDZ domains and interact, via the sixth PDZ domain (class II) with the C termini of various proteins, including liprin-alpha. In addition the PDZ456 domain mediates the, formation of homo- and heteromultimers of GRIP proteins. To better, understand the structural basis of peptide recognition by a class II PDZ, domain and PDZ-mediated multimerization, we determined the crystal, structures of the GRIP1 PDZ6 domain alone and in complex with a synthetic, C-terminal octapeptide of human liprin-alpha at resolutions of 1.5 and 1.8, A, respectively. Remarkably, unlike other class II PDZ domains, Ile-736 at, alphaB5 rather than conserved Leu-732 at alphaB1 makes a direct, hydrophobic contact with the side chain of the Tyr at the -2 position of, the ligand. Moreover, the peptide-bound structure of PDZ6 shows a slight, reorientation of helix alphaB, indicating that the second hydrophobic, pocket undergoes a conformational adaptation to accommodate the bulkiness, of the Tyr side chain, and forms an antiparallel dimer through an, interface located at a site distal to the peptide-binding groove. This, configuration may enable formation of GRIP multimers and efficient, clustering of GRIP-binding proteins.

About this Structure

1N7E is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Crystal structure of GRIP1 PDZ6-peptide complex reveals the structural basis for class II PDZ target recognition and PDZ domain-mediated multimerization., Im YJ, Park SH, Rho SH, Lee JH, Kang GB, Sheng M, Kim E, Eom SH, J Biol Chem. 2003 Mar 7;278(10):8501-7. Epub 2002 Dec 18. PMID:12493751

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