1nat
From Proteopedia
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CRYSTAL STRUCTURE OF SPOOF FROM BACILLUS SUBTILIS
Overview
Spo0F is a secondary messenger in the "two-component" system controlling, the sporulation of Bacillus subtilis. Spo0F, like the chemotaxis protein, CheY, is a single-domain protein homologous to the N-terminal activator, domain of the response regulators. We recently reported the crystal, structure of a phosphatase-resistant mutant Y13S of Spo0F with Ca2+ bound, in the active site. The crystal structure of wild-type Spo0F in the, absence of a metal ion is presented here. A comparison of the two, structures reveals that the cation induces significant changes in the, active site. In the present wild-type structure, the carboxylate of Asp11, points away from the center of the active site, whereas when coordinated, to the Ca2+, as in the earlier structure, it points toward the active, site. In addition, Asp54, the site of phosphorylation, is blocked by a, salt bridge interaction of an Arg side chain from a neighboring molecule., From fluorescence quenching studies with Spo0F Y13W, we found that only, the amino acid Arg binds to Spo0F in a saturable manner (Kd = 15 mM). This, observation suggests that a small molecule with a shape complementary to, the active site and having a guanidinium group might inhibit, phosphotransfer between response regulators and their cognate histidine, kinases.
About this Structure
1NAT is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
A response regulatory protein with the site of phosphorylation blocked by an arginine interaction: crystal structure of Spo0F from Bacillus subtilis., Madhusudan M, Zapf J, Hoch JA, Whiteley JM, Xuong NH, Varughese KI, Biochemistry. 1997 Oct 21;36(42):12739-45. PMID:9335530
Page seeded by OCA on Tue Nov 20 22:06:14 2007
