1ng7

From Proteopedia

The Solution Structure of the Soluble Domain of Poliovirus 3A Protein

Overview

Poliovirus is a positive-strand RNA virus and the prototypical member of, the Picornaviridae family. Upon infection, the viral RNA genome is, translated from a single open reading frame into a polypeptide which, undergoes a series of cleavages to ultimately form four structural and, seven non-structural proteins. A replication complex is then formed which, replicates the viral genome into negative and positive strands for further, translation, replication, and packaging into viral progeny. Poliovirus 3A, protein (3A) is a critical component of the viral replication complex and, is the putative target of enviroxime, an antiviral drug shown to block, viral replication. 3A also inhibits host cell endoplasmic, reticulum-to-Golgi apparatus transport, a function which may play a key, role in viral evasion from the host immune response. 3A, an 87-residue, protein consisting of a soluble N terminus and a hydrophobic C terminus, is formed by the cleavage of the precursor protein 3AB into 3A and 3B, (VPg). Although they differ by only 22 residues, the precursor protein 3AB, and its cleavage product 3A have distinct functions in viral replication., We have determined the structure of the soluble, N-terminal domain of 3A, (3A-N) using NMR spectroscopy. We show that 3A-N exists as a symmetric, dimer, and each monomer consists of an alpha-helical hairpin with, unstructured, yet functional, N- and C termini. We also show that the 3A-N, structure contains a negatively charged surface patch and provides a, context for interpreting the biochemical characteristics of a number of, previously reported 3A and 3AB mutants.

About this Structure

1NG7 is a Single protein structure of sequence from Human poliovirus 1. Full crystallographic information is available from OCA.

Reference

Towards an understanding of the poliovirus replication complex: the solution structure of the soluble domain of the poliovirus 3A protein., Strauss DM, Glustrom LW, Wuttke DS, J Mol Biol. 2003 Jul 4;330(2):225-34. PMID:12823963

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