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1nhy
From Proteopedia
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Crystal Structure of the GST-like Domain of Elongation Factor 1-gamma from Saccharomyces cerevisiae.
Overview
The crystal structure of the N-terminal 219 residues (domain 1) of the, conserved eukaryotic translation elongation factor 1Bgamma (eEF1Bgamma), encoded by the TEF3 gene in Saccharomyces cerevisiae, has been determined, at 3.0 A resolution by the single wavelength anomalous dispersion, technique. The structure is overall very similar to the glutathione, S-transferase proteins and contains a pocket with architecture highly, homologous to what is observed in glutathione S-transferase enzymes. The, TEF3-encoded form of eEF1Bgamma has no obvious catalytic residue. However, the second form of eEF1Bgamma encoded by the TEF4 gene contains serine 11, which may act catalytically. Based on the x-ray structure and gel, filtration studies, we suggest that the yeast eEF1 complex is organized as, an [eEF1A.eEF1Balpha.eEF1Bgamma]2 complex. A 23-residue sequence in the, middle of eEF1Bgamma is essential for the stable dimerization of, eEF1Bgamma and the quaternary structure of the eEF1 complex.
About this Structure
1NHY is a Single protein structure of sequence from Saccharomyces cerevisiae with SO4 as ligand. Full crystallographic information is available from OCA.
Reference
The crystal structure of the glutathione S-transferase-like domain of elongation factor 1Bgamma from Saccharomyces cerevisiae., Jeppesen MG, Ortiz P, Shepard W, Kinzy TG, Nyborg J, Andersen GR, J Biol Chem. 2003 Nov 21;278(47):47190-8. Epub 2003 Sep 12. PMID:12972429
Page seeded by OCA on Tue Nov 20 22:16:29 2007
