1nks
From Proteopedia
|
ADENYLATE KINASE FROM SULFOLOBUS ACIDOCALDARIUS
Overview
The adenylate kinase from the hyperthermophilic archaean species, Sulfolobus acidocaldarius has been cloned, expressed in Escherichia coli, purified and crystallized. The crystal structure was elucidated by, multiple isomorphous replacement and non-crystallographic density, averaging. The structure was refined at 2.6 A (1 A=0.1 nm) resolution. The, enzyme is trimeric, in contrast to previous solution measurements that, suggested a dimeric structure, and in contrast to the vast majority of, adenylate kinases, which are monomeric. In large parts of each subunit the, chain fold resembles the known enzyme structure from eubacteria and, eukaryotes although the sequence homology is negligible. Since the, asymmetric unit contains two trimers with and without bound AMP at the AMP, sites and with an ADP at one of the six ATP sites, the analysis shows the, enzyme in several states. The conformational differences between these, states resemble those of other adenylate kinases. Because of sequence, homology, the structure presented provides a good model for the, methanococcal adenylate kinases.
About this Structure
1NKS is a Single protein structure of sequence from Sulfolobus acidocaldarius with AMP and ADP as ligands. Active as Adenylate kinase, with EC number 2.7.4.3 Full crystallographic information is available from OCA.
Reference
The structure of a trimeric archaeal adenylate kinase., Vonrhein C, Bonisch H, Schafer G, Schulz GE, J Mol Biol. 1998 Sep 11;282(1):167-79. PMID:9733648
Page seeded by OCA on Tue Nov 20 22:20:30 2007
