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1nmf
From Proteopedia
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MAJOR COLD-SHOCK PROTEIN, NMR, 20 STRUCTURES
Overview
The cold-shock domain (CSD) is found in many eukaryotic transcriptional, factors and is responsible for the specific binding to DNA of a, cis-element called the Y-box. The same domain exists in the sequence of, the Xenopus RNA-binding proteins FRG Y1 and FRG Y2 (refs 1, 3). The major, cold-shock proteins of Escherichia coli (CS7.4) and B. subtilis (CspB), have sequences that are more than 40 per cent identical to the cold-shock, domain. We present here the three-dimensional structure of CspB determined, by nuclear magnetic resonance spectroscopy. The 67-residue protein, consists of an antiparallel five-stranded beta-barrel with strands, connected by turns and loops. The structure resembles that of, staphylococcal nuclease and the gene-5 single-stranded-DNA-binding, protein. A three-stranded beta-sheet, which contains the conserved, RNA-binding motif RNP1 as well as a motif similar to RNP2 in two, neighbouring antiparallel beta-strands, has basic and aromatic residues at, its surface which could serve as a binding site for single-stranded DNA., CspB binds to single-stranded DNA in gel retardation experiments.
About this Structure
1NMF is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
Structure in solution of the major cold-shock protein from Bacillus subtilis., Schnuchel A, Wiltscheck R, Czisch M, Herrler M, Willimsky G, Graumann P, Marahiel MA, Holak TA, Nature. 1993 Jul 8;364(6433):169-71. PMID:8321289
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