3grt
From Proteopedia
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HUMAN GLUTATHIONE REDUCTASE A34E, R37W MUTANT, OXIDIZED TRYPANOTHIONE COMPLEX
Overview
The substrate specificity of the human enzyme glutathione reductase was, changed from its natural substrate glutathione to trypanothione, [N1,N8-bis(glutathionyl)spermidine] by site-directed mutagenesis of two, residues. The glutathione analogue, trypanothione, is the natural, substrate for trypanothione reductase, an enzyme found in trypanosomatids, and leishmanias, the causative agents of diseases such as African sleeping, sickness, Chagas disease, and Oriental sore. The rational bases for our, mutational experiments were the availability of a high-resolution X-ray, structure for human glutathione reductase with bound substrates, the, active site sequence comparisons of human glutathione reductase and the, trypanothione reductases from Trypanosoma congolense and Trypanosoma, cruzi, a ... [(full description)]
About this Structure
3GRT is a [Single protein] structure of sequence from [Homo sapiens] with FAD and TS2 as [ligands]. Active as [[1]], with EC number [1.6.4.2]. Full crystallographic information is available from [OCA].
Reference
Redox enzyme engineering: conversion of human glutathione reductase into a trypanothione reductase., Bradley M, Bucheler US, Walsh CT, Biochemistry. 1991 Jun 25;30(25):6124-7. PMID:2059620
Page seeded by OCA on Mon Oct 29 20:37:00 2007
