1nzj
From Proteopedia
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Crystal Structure and Activity Studies of Escherichia Coli Yadb ORF
Overview
In the course of a structural genomics program aiming at solving the, structures of Escherichia coli open reading frame products of unknown, function, we have determined the structure of YadB at 1.5A using molecular, replacement. The YadB protein is 298 amino acid residues long and displays, 34% sequence identity with E.coli glutamyl-tRNA synthetase (GluRS). It is, much shorter than GluRS, which contains 468 residues, and lacks the, complete domain interacting with the tRNA anticodon loop. As E.coli GluRS, YadB possesses a Zn2+ located in the putative tRNA acceptor stem-binding, domain. The YadB cluster uses cysteine residues as the first three zinc, ligands, but has a weaker tyrosine ligand at the fourth position. It, shares with canonical amino acid RNA synthetases a major functional, feature, namely activation of the amino acid (here glutamate). It differs, however, from GluRSs by the fact that the activation step is, tRNA-independent and that it does not catalyze attachment of the activated, glutamate to E.coli tRNAGlu, but to another, as yet unknown tRNA. These, results suggest thus a novel function, distinct from that of GluRSs, for, the yadB gene family.
About this Structure
1NZJ is a Single protein structure of sequence from Escherichia coli with ZN as ligand. Full crystallographic information is available from OCA.
Reference
The Escherichia coli YadB gene product reveals a novel aminoacyl-tRNA synthetase like activity., Campanacci V, Dubois DY, Becker HD, Kern D, Spinelli S, Valencia C, Pagot F, Salomoni A, Grisel S, Vincentelli R, Bignon C, Lapointe J, Giege R, Cambillau C, J Mol Biol. 2004 Mar 19;337(2):273-83. PMID:15003446
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