1o29
From Proteopedia
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Crystal structure of Thymidylate Synthase Complementing Protein (TM0449) from Thermotoga maritima with FAD and FdUMP at 2.0 A resolution
Overview
Like thymidylate synthase (TS) in eukaryotes, the thymidylate, synthase-complementing proteins (TSCPs) are mandatory for cell survival of, many prokaryotes in the absence of external sources of thymidylate., Details of the mechanism of this novel family of enzymes are unknown., Here, we report the structural and functional analysis of a TSCP from, Thermotoga maritima and its complexes with substrate, analogs, and, cofactor. The structures presented here provide a basis for rationalizing, the TSCP catalysis and reveal the possibility of the design of an, inhibitor. We have identified a new helix-loop-strand FAD binding motif, characteristic of the enzymes in the TSCP family. The presence of a, hydrophobic core with residues conserved among the TSCP family suggests a, common overall fold.
About this Structure
1O29 is a Single protein structure of sequence from Thermotoga maritima with FAD and UFP as ligands. Full crystallographic information is available from OCA.
Reference
Functional analysis of substrate and cofactor complex structures of a thymidylate synthase-complementing protein., Mathews II, Deacon AM, Canaves JM, McMullan D, Lesley SA, Agarwalla S, Kuhn P, Structure. 2003 Jun;11(6):677-90. PMID:12791256
Page seeded by OCA on Tue Nov 20 22:45:03 2007
Categories: Single protein | Thermotoga maritima | Agarwalla, S. | Canaves, J.M. | Deacon, A.M. | JCSG, Joint.Center.for.Structural.Genomics. | Kuhn, P. | Lesley, S.A. | Mathews, I.I. | McMullan, D. | FAD | UFP | Jcsg | Joint center for structural genomics | Protein structure initiative | Psi | Structural genomics | Thymidylate synthase complementing protein | Tm0449
