This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1omz
From Proteopedia
|
crystal structure of mouse alpha-1,4-N-acetylhexosaminyltransferase (EXTL2) in complex with UDPGalNAc
Overview
EXTL2, an alpha1,4-N-acetylhexosaminyltransferase, catalyzes the transfer, reaction of N-acetylglucosamine and N-acetylgalactosamine from the, respective UDP-sugars to the non-reducing end of [glucuronic, acid]beta1-3[galactose]beta1-O-naphthalenemethanol, an acceptor substrate, analog of the natural common linker of various glycosylaminoglycans. We, have solved the x-ray crystal structure of the catalytic domain of mouse, EXTL2 in the apo-form and with donor substrates UDP-N-acetylglucosamine, and UDP-N-acetylgalactosamine. In addition, a structure of the ternary, complex with UDP and the acceptor substrate analog [glucuronic, acid]beta1-3[galactose]beta1-O-naphthalenemethanol has been determined., These structures reveal three highly conserved residues, Asn-243, Asp-246, and Arg-293, located at the active site. Mutation of these residues, greatly decreases the activity. In the ternary complex, an interaction, exists between the beta-phosphate of the UDP leaving group and the, acceptor hydroxyl of the substrate that may play a functional role in, catalysis. These structures represent the first structures from the, exostosin gene family and provide important insight into the mechanisms of, alpha1,4-N-acetylhexosaminyl transfer in heparan biosynthesis.
About this Structure
1OMZ is a Single protein structure of sequence from Mus musculus with MN, UD2 and EDO as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of an alpha 1,4-N-acetylhexosaminyltransferase (EXTL2), a member of the exostosin gene family involved in heparan sulfate biosynthesis., Pedersen LC, Dong J, Taniguchi F, Kitagawa H, Krahn JM, Pedersen LG, Sugahara K, Negishi M, J Biol Chem. 2003 Apr 18;278(16):14420-8. Epub 2003 Jan 31. PMID:12562774
Page seeded by OCA on Tue Nov 20 23:01:19 2007
Categories: Mus musculus | Single protein | Dong, J. | Kitagawa, H. | Krahn, J.M. | Negishi, M. | Pedersen, L.C. | Pedersen, L.G. | Sugahara, K. | Taniguchi, F. | EDO | MN | UD2 | Dxd motif | Rossmann fold
