This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1orc
From Proteopedia
|
CRO REPRESSOR INSERTION MUTANT K56-[DGEVK]
Overview
A rationally designed, genetically engineered, monomeric form of the Cro, protein from bacteriophage lambda has been crystallized and its structure, determined by isomorphous replacement and refined to a resolution of 1.54, A. The structure confirms the rationale of the design but, at the same, time, reveals 1-2 A shifts throughout the monomer structure relative to, the previously determined structure of the dimeric wild-type protein., These changes include a 1.6 A main-chain shift in part of the beta-sheet, region of the molecule relative to the alpha-helical region and a 1.1 A, shift of a buried phenylalanine within the core as well as a correlated, 2.2 A shift in a solvent-exposed beta-hairpin. The conformational, adjustments appear to reflect an inherent flexibility of the protein that, is associated with its DNA-binding function.
About this Structure
1ORC is a Single protein structure of sequence from Enterobacteria phage lambda. Full crystallographic information is available from OCA.
Reference
High-resolution structure of an engineered Cro monomer shows changes in conformation relative to the native dimer., Albright RA, Mossing MC, Matthews BW, Biochemistry. 1996 Jan 23;35(3):735-42. PMID:8547253
Page seeded by OCA on Tue Nov 20 23:07:33 2007
