2v0o
From Proteopedia
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FCHO2 F-BAR DOMAIN
Overview
A spectrum of membrane curvatures exists within cells, and proteins have, evolved different modules to detect, create, and maintain these, curvatures. Here we present the crystal structure of one such module found, within human FCHo2. This F-BAR (extended FCH) module consists of two F-BAR, domains, forming an intrinsically curved all-helical antiparallel dimer, with a K(d) of 2.5 muM. The module binds liposomes via a concave face, deforming them into tubules with variable diameters of up to 130 nm. Pulse, EPR studies showed the membrane-bound dimer is the same as the crystal, dimer, although the N-terminal helix changed conformation on membrane, binding. Mutation of a phenylalanine on this helix partially attenuated, narrow tubule formation, and resulted in a gain of curvature ... [(full description)]
About this Structure
2V0O is a [Single protein] structure of sequence from [Homo sapiens] with ACT as [ligand]. Full crystallographic information is available from [OCA].
Reference
Structure and Analysis of FCHo2 F-BAR Domain: A Dimerizing and Membrane Recruitment Module that Effects Membrane Curvature., Henne WM, Kent HM, Ford MG, Hegde BG, Daumke O, Butler PJ, Mittal R, Langen R, Evans PR, McMahon HT, Structure. 2007 Jul;15(7):839-52. Epub 2007 Jun 1. PMID:17540576
Page seeded by OCA on Mon Oct 29 20:42:34 2007
