1paa
From Proteopedia
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STRUCTURE OF A HISTIDINE-X4-HISTIDINE ZINC FINGER DOMAIN: INSIGHTS INTO ADR1-UAS1 PROTEIN-DNA RECOGNITION
Overview
The solution structure for a mutant zinc finger peptide based on the, sequence of the C-terminal ADR1 finger has been determined by, two-dimensional NMR spectroscopy. The mutant peptide, called PAPA, has, both proline residues from the wild-type sequence replaced with alanines., A nonessential cysteine was also replaced with alanine. The behavior of, PAPA in solution implicates the prolines in the conformational, heterogeneity reported earlier for the wild-type peptide [Xu, R. X., Horvath, S. J., & Klevit, R. E. (1991) Biochemistry 30, 3365-3371]. The, solution structure of PAPA reveals several interesting features of the, zinc finger motif. The residue immediately following the second cysteine, ligand adopts a positive phi angle, which we propose is a common feature, of this class of zinc fingers, regardless of whether this residue is a, glycine. The NMR spectrum and resulting solution structure of PAPA suggest, that a side-chain to side-chain hydrogen bond involving an arginine and an, aspartic acid analogous to one observed in the Zif268 protein-DNA, cocrystal structure exists in solution in the absence of DNA [Pavletich, N. P., & Pabo, C. O. (1991) Science 252, 809-817]. A model for the, interaction between the two ADR1 zinc fingers and their DNA binding sites, was built by superpositioning the refined solution structures of PAPA and, ADR1b onto the Zif268 structure. This model offers structural explanations, for a variety of mutations to the ADR1 zinc finger domains that have been, shown to affect DNA-binding affinity or specificity.
About this Structure
1PAA is a Single protein structure of sequence from Saccharomyces cerevisiae with ZN as ligand. Full crystallographic information is available from OCA.
Reference
Structure of a histidine-X4-histidine zinc finger domain: insights into ADR1-UAS1 protein-DNA recognition., Bernstein BE, Hoffman RC, Horvath S, Herriott JR, Klevit RE, Biochemistry. 1994 Apr 19;33(15):4460-70. PMID:8161501
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