1paz
From Proteopedia
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REFINEMENT OF THE STRUCTURE OF PSEUDOAZURIN FROM ALCALIGENES FAECALIS S-6 AT 1.55 ANGSTROMS RESOLUTION
Overview
The crystal structure of the redox protein pseudoazurin (123 amino acid, residues; molecular weight 13,000 daltons) from Alcaligenes faecalis has, been refined by fast Fourier restrained least-squares minimization. Cycles, of rebuilding were carried out to escape from local minima. Individual, isotropic temperature factor values were refined separately for all atoms., The R factor was reduced from 0.400 (for 2647 reflections in the 6.0-2.8 A, resolution range) to 0.180 (for all 19,770 reflections in the 9.0-1.55 A, resolution range) with a final estimated accuracy in atomic positions of, 0.15 A. The final model comprises 917 protein atoms and 93 solvent, molecules. The root-mean-square shift of the main-chain atoms between the, final and the initial model is 0.94 A (maximum shift 1.8 A). Most of the, larger shifts were the result of rebuilding on the graphics system. The, average atomic temperature factor, B, is 23.0 A2 for all atoms. Side-chain, atoms with high B values were omitted, and their positions checked from, difference maps. The three carboxy-terminal residues were omitted from the, final model as no single conformation could be assigned from the observed, electron density. All other protein atoms were included.
About this Structure
1PAZ is a Single protein structure of sequence from Alcaligenes faecalis with CU as ligand. Full crystallographic information is available from OCA.
Reference
Refinement of the structure of pseudoazurin from Alcaligenes faecalis S-6 at 1.55 A resolution., Petratos K, Dauter Z, Wilson KS, Acta Crystallogr B. 1988 Dec 1;44 ( Pt 6):628-36. PMID:3271558
Page seeded by OCA on Tue Nov 20 23:38:54 2007
