1w08

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spinqualitylabelsall models 1w08, resolution 2.5Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

STRUCTURE OF T70N HUMAN LYSOZYME

Overview

T70N human lysozyme is the only known naturally occurring destabilised, lysozyme variant that has not been detected in amyloid deposits in human, patients. Its study and a comparison of its properties with those of the, amyloidogenic variants of lysozyme is therefore important for, understanding the determinants of amyloid disease. We report here the, X-ray crystal structure and the solution dynamics of T70N lysozyme, as, monitored by hydrogen/deuterium exchange and NMR relaxation experiments., The X-ray crystal structure shows that a substantial structural, rearrangement results from the amino acid substitution, involving residues, 45-51 and 68-75 in particular, and gives rise to a concomitant separation, of these two loops of up to 6.5A. A marked decrease in the magnitudes of, the ... [(full description)]

About this Structure

1W08 is a [Single protein] structure of sequence from [Homo sapiens] with CL as [ligand]. Active as [[1]], with EC number [3.2.1.17]. Full crystallographic information is available from [OCA].

Reference

Rationalising lysozyme amyloidosis: insights from the structure and solution dynamics of T70N lysozyme., Johnson RJ, Christodoulou J, Dumoulin M, Caddy GL, Alcocer MJ, Murtagh GJ, Kumita JR, Larsson G, Robinson CV, Archer DB, Luisi B, Dobson CM, J Mol Biol. 2005 Sep 30;352(4):823-36. PMID:16126226

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