1w0k
From Proteopedia
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BERYLLIUM FLUORIDE INHIBITED BOVINE F1-ATPASE
Overview
The structure of bovine F1-ATPase inhibited with ADP and beryllium, fluoride at 2.0 angstroms resolution contains two ADP.BeF3- complexes, mimicking ATP, bound in the catalytic sites of the beta(TP) and beta(DP), subunits. Except for a 1 angstrom shift in the guanidinium of alphaArg373, the conformations of catalytic side chains are very similar in both sites., However, the ordered water molecule that carries out nucleophilic attack, on the gamma-phosphate of ATP during hydrolysis is 2.6 angstroms from the, beryllium in the beta(DP) subunit and 3.8 angstroms away in the beta(TP), subunit, strongly indicating that the beta(DP) subunit is the, catalytically active conformation. In the structure of F1-ATPase with five, bound ADP molecules (three in alpha-subunits, one each in the beta(TP) ... [(full description)]
About this Structure
1W0K is a [Protein complex] structure of sequences from [Bos taurus] with MG, PO4, ADP and GOL as [ligands]. Active as [[1]], with EC number [3.6.3.14]. Full crystallographic information is available from [OCA].
Reference
The structure of bovine F1-ATPase inhibited by ADP and beryllium fluoride., Kagawa R, Montgomery MG, Braig K, Leslie AG, Walker JE, EMBO J. 2004 Jul 21;23(14):2734-44. Epub 2004 Jul 1. PMID:15229653
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