1pkv
From Proteopedia
|
The N-terminal domain of riboflavin synthase in complex with riboflavin
Overview
Riboflavin synthase of Escherichia coli is a homotrimer with a molecular, mass of 70 kDa. The enzyme catalyzes the dismutation of, 6,7-dimethyl-8-(1'-D-ribityl)-lumazine, affording riboflavin and, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione. The N-terminal segment, (residues 1-87) and the C-terminal segment (residues 98-187) form, beta-barrels with similar fold and a high degree of sequence similarity. A, recombinant peptide comprising amino acid residues 1-97 forms a dimer, which binds riboflavin with high affinity. Here, we report the structure, of this construct in complex with riboflavin at 2.6A resolution. It is, demonstrated that the complex can serve as a model for ligand-binding in, the native enzyme. The structure and riboflavin-binding mode is in, excellent agreement with structural information obtained from the native, enzyme from Escherichia coli and riboflavin synthase from, Schizosaccharomyces pombe. The implications for the binding specificity, and the regiospecificity of the catalyzed reaction are discussed.
About this Structure
1PKV is a Single protein structure of sequence from Escherichia coli with RBF as ligand. Active as Riboflavin synthase, with EC number 2.5.1.9 Full crystallographic information is available from OCA.
Reference
The structure of the N-terminal domain of riboflavin synthase in complex with riboflavin at 2.6A resolution., Meining W, Eberhardt S, Bacher A, Ladenstein R, J Mol Biol. 2003 Aug 29;331(5):1053-63. PMID:12927541
Page seeded by OCA on Tue Nov 20 23:54:01 2007
