1put
From Proteopedia
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AN NMR-DERIVED MODEL FOR THE SOLUTION STRUCTURE OF OXIDIZED PUTIDAREDOXIN, A 2FE, 2-S FERREDOXIN FROM PSEUDOMONAS
Overview
A model for the solution structure of oxidized putidaredoxin (Pdx), a, 106-residue globular protein containing a Fe2S2 cluster, has been, determined using homonuclear NMR methods. Pdx is the first of the class of, Fe2S2Cys4 ferredoxins which act as electron-transfer partners for P-450, monooxygenases to be structurally characterized, and no crystal structure, has been determined for Pdx or for any closely homologous protein. Pdx is, the physiological redox partner of cytochrome P-450cam. A total of 878 NOE, distance constraints, 66 phi angular constraints derived from NH-C alpha H, coupling constants, and five paramagnetic broadening constraints were used, in simulated annealing structural refinements to obtain a family of, structures with pairwise rms deviations of 1.14 A for backbone atoms and, 1.80 A for all non-hydrogen atoms. Paramagnetic broadening of resonances, within a ca. 8-A radius of the metal cluster prevents the use of, NMR-derived constraints in this region of the protein; structural, constraints used to model the environment of the metal cluster were, obtained from site-directed mutagenesis and model compounds and by, comparison with known ferredoxin structures. Pdx retains a similar folding, topology to other structurally characterized Fe2S2Cys4 ferredoxins but, differs from the other ferredoxins in containing a significantly more, compact structure in the C-terminal half of the protein.
About this Structure
1PUT is a Single protein structure of sequence from Pseudomonas putida with FES as ligand. Full crystallographic information is available from OCA.
Reference
An NMR-derived model for the solution structure of oxidized putidaredoxin, a 2-Fe, 2-S ferredoxin from Pseudomonas., Pochapsky TC, Ye XM, Ratnaswamy G, Lyons TA, Biochemistry. 1994 May 31;33(21):6424-32. PMID:8204575
Page seeded by OCA on Wed Nov 21 00:08:22 2007
