1py0
From Proteopedia
|
Crystal structure of E51C/E54C Psaz from A.faecalis with CLaNP probe
Overview
A lanthanide complex, named CLaNP (caged lanthanide NMR probe) has been, developed for the characterisation of proteins by paramagnetic NMR, spectroscopy. The probe consists of a lanthanide chelated by a derivative, of DTPA (diethylenetriaminepentaacetic acid) with two thiol reactive, functional groups. The CLaNP molecule is attached to a protein by two, engineered, surface-exposed, Cys residues in a bidentate manner. This, drastically limits the dynamics of the metal relative to the protein and, enables measurements of pseudocontact shifts. NMR spectroscopy experiments, on a diamagnetic control and the crystal structure of the probe-protein, complex demonstrate that the protein structure is not affected by probe, attachment. The probe is able to induce pseudocontact shifts to at least, 40 A from the metal and causes residual dipolar couplings due to alignment, at a high magnetic field. The molecule exists in several isomeric forms, with different paramagnetic tensors; this provides a fast way to obtain, long-range distance restraints.
About this Structure
1PY0 is a Single protein structure of sequence from Alcaligenes faecalis with ZN, Y1, SO4 and YMA as ligands. Full crystallographic information is available from OCA.
Reference
A caged lanthanide complex as a paramagnetic shift agent for protein NMR., Prudencio M, Rohovec J, Peters JA, Tocheva E, Boulanger MJ, Murphy ME, Hupkes HJ, Kosters W, Impagliazzo A, Ubbink M, Chemistry. 2004 Jul 5;10(13):3252-60. PMID:15224334
Page seeded by OCA on Wed Nov 21 00:13:24 2007
Categories: Alcaligenes faecalis | Single protein | Boulanger, M.J. | Hupkes, H.J. | Impagliazzo, A. | Kosters, W. | Murphy, M.E. | Peters, J.A. | Prudencio, M. | Rohovec, J. | Tocheva, E. | Ubbink, M. | SO4 | Y1 | YMA | ZN | Cupredoxin | Nmr probe
