1q9y
From Proteopedia
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CRYSTAL STRUCTURE OF ENTEROBACTERIA PHAGE RB69 GP43 DNA POLYMERASE COMPLEXED WITH 8-OXOGUANOSINE CONTAINING DNA
Overview
The initial encounter of an unrepaired DNA lesion is likely to be with a, replicative DNA polymerase, and the outcome of this event determines, whether an error-prone or error-free damage avoidance pathway is taken. To, understand the atomic details of this critical encounter, we have, determined the crystal structures of the pol alpha family RB69 DNA, polymerase with DNA containing the two most prevalent, spontaneously, generated premutagenic lesions, an abasic site and, 2'-deoxy-7,8-dihydro-8-oxoguanosine (8-oxodG). Identification of the, interactions between these damaged nucleotides and the active site, provides insight into the capacity of the polymerase to incorporate a base, opposite the lesion. A novel open, catalytically inactive conformation of, the DNA polymerase has been identified in the complex with a primed abasic, site template. This structure provides the first molecular, characterization of the DNA synthesis barrier caused by an abasic site and, suggests a general mechanism for polymerase fidelity. In contrast, the, structure of the ternary 8-oxodG:dCTP complex is almost identical to the, replicating complex containing unmodified DNA, explaining the relative, ease and fidelity by which this lesion is bypassed.
About this Structure
1Q9Y is a Single protein structure of sequence from Enterobacteria phage rb18 with CA and DCP as ligands. Active as DNA-directed DNA polymerase, with EC number 2.7.7.7 Full crystallographic information is available from OCA.
Reference
Lesion (in)tolerance reveals insights into DNA replication fidelity., Freisinger E, Grollman AP, Miller H, Kisker C, EMBO J. 2004 Apr 7;23(7):1494-505. Epub 2004 Apr 1. PMID:15057282
Page seeded by OCA on Wed Nov 21 00:30:50 2007
