1qld
From Proteopedia
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SOLUTION STRUCTURE OF TYPE X CBM
Overview
Plant cell wall hydrolases generally have a modular structure consisting, of a catalytic domain linked to one or more noncatalytic, carbohydrate-binding modules (CBMs), whose common function is to attach, the enzyme to the polymeric substrate. Xylanase A from Pseudomonas, fluorescens subsp. cellulosa (Pf Xyn10A) consists of a family 10 catalytic, domain, an N-terminal family IIa cellulose-binding module, and an internal, family 10 cellulose-binding module. The structure of the 45-residue family, 10 CBM has been determined in solution using NMR. It consists of two, antiparallel beta-sheets, one with two strands and one with three, with a, short alpha-helix across one face of the three-stranded sheet. There is a, high density of aromatic residues on one side of the protein, including, three aromatic residues (Tyr8, Trp22, and Trp24), which are exposed and, form a flat surface on one face, in a classical polysaccharide-binding, arrangement. The fold is closely similar to that of the, oligonucleotide/oligosaccharide-binding (OB) fold, but appears to have, arisen by convergent evolution, because there is no sequence similarity, and the presumed binding sites are on different faces.
About this Structure
1QLD is a Single protein structure of sequence from Pseudomonas fluorescens. Active as Endo-1,4-beta-xylanase, with EC number 3.2.1.8 Full crystallographic information is available from OCA.
Reference
Solution structure of the CBM10 cellulose binding module from Pseudomonas xylanase A., Raghothama S, Simpson PJ, Szabo L, Nagy T, Gilbert HJ, Williamson MP, Biochemistry. 2000 Feb 8;39(5):978-84. PMID:10653641
Page seeded by OCA on Wed Nov 21 00:47:32 2007
