1r20

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spinqualitylabelsall models 1r20, resolution 3.0Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of the ligand-binding domains of the heterodimer EcR/USP bound to the synthetic agonist BYI06830

Overview

The ecdysteroid hormones coordinate the major stages of insect, development, notably moulting and metamorphosis, by binding to the, ecdysone receptor (EcR); a ligand-inducible nuclear transcription factor., To bind either ligand or DNA, EcR must form a heterodimer with, ultraspiracle (USP), the homologue of retinoid-X receptor. Here we report, the crystal structures of the ligand-binding domains of the moth Heliothis, virescens EcR-USP heterodimer in complex with the ecdysteroid ponasterone, A and with a non-steroidal, lepidopteran-specific agonist BYI06830 used in, agrochemical pest control. The two structures of EcR-USP emphasize the, universality of heterodimerization as a general mechanism common to both, vertebrates and invertebrates. Comparison of the EcR structures in complex, with steroidal and non-steroidal ligands reveals radically different and, only partially overlapping ligand-binding pockets that could not be, predicted by molecular modelling and docking studies. These findings offer, new perspectives for the design of insect-specific, environmentally safe, insecticides. The concept of a ligand-dependent binding pocket in EcR, provides an insight into the moulding of nuclear receptors to their, ligand, and has potential applications for human nuclear receptors.

About this Structure

1R20 is a Protein complex structure of sequences from Heliothis virescens with HWG and EPH as ligands. Full crystallographic information is available from OCA.

Reference

Structural adaptability in the ligand-binding pocket of the ecdysone hormone receptor., Billas IM, Iwema T, Garnier JM, Mitschler A, Rochel N, Moras D, Nature. 2003 Nov 6;426(6962):91-6. Epub 2003 Nov 2. PMID:14595375

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