1r48
From Proteopedia
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Solution structure of the C-terminal cytoplasmic domain residues 468-497 of Escherichia coli protein ProP
Overview
Bacteria respond to increasing medium osmolality by accumulating organic, solutes that are compatible with cellular functions. Transporter ProP of, Escherichia coli, a proton symporter and a member of the major facilitator, superfamily, senses osmotic shifts and responds by importing osmolytes, such as glycine betaine. ProP contains a cytoplasmic, C-terminal extension, that is essential for its activity. A peptide corresponding to the, C-terminal extension of ProP forms a homodimeric alpha-helical coiled-coil, even though some of its heptad a positions are not occupied by hydrophobic, amino acid residues. Unexpectedly, amino acid replacement R488I, occurring, at a heptad a position, destabilized the coiled-coil formed by the ProP, peptide and attenuated the response of the intact transporter to osmotic, upshifts in vivo. Thus, ProP was proposed to dimerize via an antiparallel, coiled-coil. We used nuclear magnetic resonance (NMR) spectroscopy to, determine the structure of the synthetic peptide corresponding to residues, 468-497 of ProP. This region did form an antiparallel coil-coil in which, critical residue R488 specifies the antiparallel coiled-coil orientation, by forming stabilizing salt-bridges. Charged residues (both acidic and, basic) are clustered on the c/g surface of the coiled-coil whereas polar, residues are distributed on the b/e surface. This causes the structure to, be bent, in contrast to other known antiparallel coiled-coils (those from, the hepatitis delta antigen (PDB ID code 1A92) and the bovine F(1) ATPase, inhibitor protein (PDB ID code 1HF9)). The coiled-coil and its possible, importance for osmosensing are discussed.
About this Structure
1R48 is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Solution structure of the C-terminal antiparallel coiled-coil domain from Escherichia coli osmosensor ProP., Zoetewey DL, Tripet BP, Kutateladze TG, Overduin MJ, Wood JM, Hodges RS, J Mol Biol. 2003 Dec 12;334(5):1063-76. PMID:14643666
Page seeded by OCA on Wed Nov 21 01:15:03 2007
