1rbn
From Proteopedia
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THE STRUCTURE OF RIBONUCLEASE A DERIVATIVE II AT 2.1 ANGSTROMS RESOLUTION
Overview
The crystal structure of bovine pancreatic ribonuclease A derivative II, a, covalent derivative obtained by reaction of 6-chloropurine, 9-beta-D-ribofuranosyl 5'-monophosphate with the alpha-amino group of, Lys-1, has been determined and refined at 2.1-A resolution with an, agreement factor R = 0.166 for 6254 reflections in the resolution shell, 8.0 to 2.1 A. Crystals are orthorhombic and belong to space group C222(1), with unit cell parameters a = 75.73 A, b = 57.85 A, and c = 53.26 A. This, crystal packing had never been reported before for pancreatic ribonuclease, nor its complexes. The structure found is in accordance with the location, of p2, B3, and R3 subsites at the N-terminal region of the protein and, provides an explanation of the catalytic behavior observed for this, derivative. In particular, differences in kinetic parameters and in the, pKa value of His-119 between derivative II and native ribonuclease A can, be interpreted on the basis of the position of the phosphate moiety within, the derivative structure. Some uncertainty remains on the nucleotide sugar, conformation determined.
About this Structure
1RBN is a Single protein structure of sequence from Bos taurus with P5P as ligand. Active as Pancreatic ribonuclease, with EC number 3.1.27.5 Full crystallographic information is available from OCA.
Reference
Structure of ribonuclease A derivative II at 2.1-A resolution., Boque L, Gracia Coll M, Vilanova M, Cuchillo CM, Fita I, J Biol Chem. 1994 Aug 5;269(31):19707-12. PMID:8051049
Page seeded by OCA on Wed Nov 21 01:26:31 2007
