1rdr
From Proteopedia
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POLIOVIRUS 3D POLYMERASE
Overview
BACKGROUND: The central player in the replication of RNA viruses is the, viral RNA-dependent RNA polymerase. The 53 kDa poliovirus polymerase, together with other viral and possibly host proteins, carries out viral, RNA replication in the host cell cytoplasm. RNA-dependent RNA polymerases, comprise a distinct category of polymerases that have limited sequence, similarity to reverse transcriptases (RNA-dependent DNA polymerases) and, perhaps also to DNA-dependent polymerases. Previously reported structures, of RNA-dependent DNA polymerases, DNA-dependent DNA polymerases and a, DNA-dependent RNA polymerase show that structural and evolutionary, relationships exist between the different polymerase categories. RESULTS:, We have determined the structure of the RNA-dependent RNA polymerase of, poliovirus at 2.6 A resolution by X-ray crystallography. It has the same, overall shape as other polymerases, commonly described by analogy to a, right hand. The structures of the 'fingers' and 'thumb' subdomains of, poliovirus polymerase differ from those of other polymerases, but the palm, subdomain contains a core structure very similar to that of other, polymerases. This conserved core structure is composed of four of the, amino acid sequence motifs described for RNA-dependent polymerases., Structure-based alignments of these motifs has enabled us to modify and, extend previous sequence and structural alignments so as to relate, sequence conservation to function. Extensive regions of, polymerase-polymerase interactions observed in the crystals suggest an, unusual higher order structure that we believe is important for polymerase, function. CONCLUSIONS: As a first example of a structure of an, RNA-dependent RNA polymerase, the poliovirus polymerase structure provides, for a better understanding of polymerase structure, function and, evolution. In addition, it has yielded insights into an unusual higher, order structure that may be critical for poliovirus polymerase function.
About this Structure
1RDR is a Single protein structure of sequence from Human poliovirus 1 with CA as ligand. Active as RNA-directed RNA polymerase, with EC number 2.7.7.48 Full crystallographic information is available from OCA.
Reference
Structure of the RNA-dependent RNA polymerase of poliovirus., Hansen JL, Long AM, Schultz SC, Structure. 1997 Aug 15;5(8):1109-22. PMID:9309225
Page seeded by OCA on Wed Nov 21 01:30:15 2007
