1rld

From Proteopedia

SOLID-STATE PHASE TRANSITION IN THE CRYSTAL STRUCTURE OF RIBULOSE 1,5-BIPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE

Overview

The crystal structure is described of ribulose 1,5-bisphosphate, carboxylase/oxygenase in a new crystal form. This new form (form V) was, obtained from a previously known crystal form (form III) through a, solid-state phase transition. The solid-state phase transition was brought, about by transferring the crystal from a high-salt low-pH mother liquor to, a low-salt high-pH synthetic mother liquor. The interplay of electrostatic, repulsion and osmotic pressure induced a unit-cell shrinkage of 24 A along, the c axis and expansion of 4 A along the a and b axes. The space group, also changed from I422 to I4. The new crystal form was shown to be more, resistant to X-ray radiation damage, which suggests the effect of crystal, stabilization by non-penetrating molecules. The structure of ribulose, 1,5-bisphosphate carboxylase/oxygenase in the new crystal form is compared, with that of the old crystal form.

About this Structure

1RLD is a Protein complex structure of sequences from Nicotiana tabacum. Active as Ribulose-bisphosphate carboxylase, with EC number 4.1.1.39 Full crystallographic information is available from OCA.

Reference

Solid-state phase transition in the crystal structure of ribulose 1,5-bisphosphate carboxylase/oxygenase., Zhang KY, Eisenberg D, Acta Crystallogr D Biol Crystallogr. 1994 May 1;50(Pt 3):258-62. PMID:15299436

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