This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1rlf
From Proteopedia
|
STRUCTURE DETERMINATION OF THE RAS-BINDING DOMAIN OF THE RAL-SPECIFIC GUANINE NUCLEOTIDE EXCHANGE FACTOR RLF, NMR, 10 STRUCTURES
Overview
Ral-specific guanine nucleotide exchange factors RalGDS, Rgl, and Rlf have, been suggested to function as intermediates between Ras and Ral pathways, by being able to bind Ras proteins through their C-terminal Ras-binding, domains (RBD). The RBDs of RalGDS and of the Ser/Thr kinase c-Raf-1 have, been shown to have the same tertiary structure. In contrast to the RBDs of, Raf and RalGDS, which bind either Ras or Rap with high affinity, Rlf-RBD, has a similar affinity for both GTP-binding proteins. To be able to, compare these RBDs on a structural level, we have solved the, three-dimensional structure of Rlf-RBD by NMR spectroscopy. The overall, tertiary structure of Rlf-RBD shows the, betabetaalphabetabetaalphabeta-fold of the ubiquitin superfamily and is, very similar to that of RalGDS-RBD. The binding interface of Rlf-RBD to, Ras was mapped using chemical shift analysis and indicated a binding mode, similar to that in the case of Rap.Raf-RBD. However, comparison of the, putatively interacting regions revealed structural differences which are, proposed to be responsible for the different substrate affinities of Rlf-, RalGDS-, and Raf-RBD.
About this Structure
1RLF is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Structure determination of the Ras-binding domain of the Ral-specific guanine nucleotide exchange factor Rlf., Esser D, Bauer B, Wolthuis RM, Wittinghofer A, Cool RH, Bayer P, Biochemistry. 1998 Sep 29;37(39):13453-62. PMID:9753431
Page seeded by OCA on Wed Nov 21 01:41:12 2007
