This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1s7z
From Proteopedia
|
Structure of Ocr from Bacteriophage T7
Overview
We have solved, by X-ray crystallography to a resolution of 1.8 A, the, structure of a protein capable of mimicking approximately 20 base pairs of, B-form DNA. This ocr protein, encoded by gene 0.3 of bacteriophage T7, mimics the size and shape of a bent DNA molecule and the arrangement of, negative charges along the phosphate backbone of B-form DNA. We also, demonstrate that ocr is an efficient inhibitor in vivo of all known, families of the complex type I DNA restriction enzymes. Using atomic force, microscopy, we have also observed that type I enzymes induce a bend in DNA, of similar magnitude to the bend in the ocr molecule. This first structure, of an antirestriction protein demonstrates the construction of structural, mimetics of long segments of B-form DNA.
About this Structure
1S7Z is a Single protein structure of sequence from Bacteriophage t7 with CS as ligand. Full crystallographic information is available from OCA.
Reference
Structure of Ocr from bacteriophage T7, a protein that mimics B-form DNA., Walkinshaw MD, Taylor P, Sturrock SS, Atanasiu C, Berge T, Henderson RM, Edwardson JM, Dryden DT, Mol Cell. 2002 Jan;9(1):187-94. PMID:11804597
Page seeded by OCA on Wed Nov 21 02:11:16 2007
