This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1sds
From Proteopedia
|
Structure of protein L7Ae bound to a K-turn derived from an archaeal box H/ACA sRNA
Overview
The archaeal RNA binding protein L7Ae and its eukaryotic homolog 15.5, kDa/Snu13 recognize K-turns. This structural motif is canonically, comprised of two stems (one with tandem A.G base pairs, the other with, Watson-Crick pairs) linked by an asymmetric internal loop. L7Ae recognizes, conventional K-turns in ribosomal and box C/D RNAs but also binds, specifically to some box H/ACA RNAs at terminal stem loops. These have the, A.G paired stem, but lack the Watson-Crick stem. The structure of, Methanococcus jannaschii L7Ae bound to a symmetric duplex RNA without, Watson-Crick stems demonstrates how a binding site for this component of, diverse ribonucleoprotein complexes can be constructed with only the A.G, stem and the loop. The RNA adopts a functional conformation with the aid, of a base triple and tight binding of divalent cations. Comparison with, the 15.5 kDa/Snu13-RNA complex structure suggests why the eukaryotic, homolog does not recognize terminal stem loop L7Ae binding sites.
About this Structure
1SDS is a Single protein structure of sequence from Methanocaldococcus jannaschii with CA and K as ligands. Full crystallographic information is available from OCA.
Reference
Structure of protein L7Ae bound to a K-turn derived from an archaeal box H/ACA sRNA at 1.8 A resolution., Hamma T, Ferre-D'Amare AR, Structure. 2004 May;12(5):893-903. PMID:15130481
Page seeded by OCA on Wed Nov 21 02:18:18 2007
