1sgv

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spinqualitylabelsall models 1sgv, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

STRUCTURE OF TRNA PSI55 PSEUDOURIDINE SYNTHASE (TRUB)

Overview

The three-dimensional structure of the RNA-modifying enzyme, psi55 tRNA, pseudouridine synthase from Mycobacterium tuberculosis, is reported. The, 1.9-A resolution crystal structure reveals the enzyme, free of substrate, in two distinct conformations. The structure depicts an interesting mode, of protein flexibility involving a hinged bending in the central, beta-sheet of the catalytic module. Key parts of the active site cleft are, also found to be disordered in the substrate-free form of the enzyme. The, hinge bending appears to act as a clamp to position the substrate. Our, structural data furthers the previously proposed mechanism of tRNA, recognition. The present crystal structure emphasizes the significant role, that protein dynamics must play in tRNA recognition, base flipping, and, modification.

About this Structure

1SGV is a Single protein structure of sequence from Mycobacterium tuberculosis. This structure superseeds the now removed PDB entry 1S71. Active as Pseudouridylate synthase, with EC number 4.2.1.70 Full crystallographic information is available from OCA.

Reference

Crystal structure of the apo forms of psi 55 tRNA pseudouridine synthase from Mycobacterium tuberculosis: a hinge at the base of the catalytic cleft., Chaudhuri BN, Chan S, Perry LJ, Yeates TO, J Biol Chem. 2004 Jun 4;279(23):24585-91. Epub 2004 Mar 17. PMID:15028724

Page seeded by OCA on Wed Nov 21 02:22:49 2007