1sv0
From Proteopedia
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Crystal Structure Of Yan-SAM/Mae-SAM Complex
Overview
Yan, an ETS family transcriptional repressor, is regulated by receptor, tyrosine kinase signaling via the Ras/MAPK pathway. Phosphorylation and, downregulation of Yan is facilitated by a protein called Mae. Yan and Mae, interact through their SAM domains. We find that repression by Yan, requires the formation of a higher order structure mediated by Yan-SAM, polymerization. Moreover, a crystal structure of the Yan-SAM/Mae-SAM, complex shows that Mae-SAM specifically recognizes a surface on Yan-SAM, that is also required for Yan-SAM polymerization. Mae-SAM binds to Yan-SAM, with approximately 1000-fold higher affinity than Yan-SAM binds to itself, and can effectively depolymerize Yan-SAM. Mutations on Mae that, specifically disrupt its SAM domain-dependent interactions with Yan, disable the derepression function of Mae in vivo. Depolymerization of Yan, by Mae represents a novel mechanism of transcriptional control that, sensitizes Yan for regulation by receptor tyrosine kinases.
About this Structure
1SV0 is a Protein complex structure of sequences from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Derepression by depolymerization; structural insights into the regulation of Yan by Mae., Qiao F, Song H, Kim CA, Sawaya MR, Hunter JB, Gingery M, Rebay I, Courey AJ, Bowie JU, Cell. 2004 Jul 23;118(2):163-73. PMID:15260987
Page seeded by OCA on Wed Nov 21 02:41:53 2007
