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1syo
From Proteopedia
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N-terminal 3 domains of CI-MPR bound to mannose 6-phosphate
Overview
The 300-kDa cation-independent mannose 6-phosphate receptor (CI-MPR) plays, a critical role in the trafficking of newly synthesized mannose, 6-phosphate-containing acid hydrolases to the lysosome. The receptor, contains two high affinity carbohydrate recognition sites within its, 15-domain extracytoplasmic region, with essential residues for, carbohydrate recognition located in domain 3 and domain 9. Previous, studies have shown that these two sites are distinct with respect to, carbohydrate specificity. In addition, expression of truncated forms of, the CI-MPR demonstrated that domain 9 can be expressed as an isolated, domain, retaining high affinity (Kd approximately 1 nm) carbohydrate, binding, whereas expression of domain 3 alone resulted in a protein, capable of only low affinity binding (Kd approximately 1 microm) toward a, lysosomal enzyme. In the current report the crystal structure of the, N-terminal 432 residues of the CI-MPR, encompassing domains 1-3, was, solved in the presence of bound mannose 6-phosphate. The structure reveals, the unique architecture of this carbohydrate binding pocket and provides, insight into the ability of this site to recognize a variety of, mannose-containing sugars.
About this Structure
1SYO is a Single protein structure of sequence from Bos taurus with NAG, M6P and GOL as ligands. Full crystallographic information is available from OCA.
Reference
The N-terminal carbohydrate recognition site of the cation-independent mannose 6-phosphate receptor., Olson LJ, Dahms NM, Kim JJ, J Biol Chem. 2004 Aug 6;279(32):34000-9. Epub 2004 May 28. PMID:15169779
Page seeded by OCA on Wed Nov 21 02:51:23 2007
