1t61

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spinqualitylabelsall models 1t61, resolution 1.5Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

crystal structure of collagen IV NC1 domain from placenta basement membrane

Overview

Collagen IV networks are present in all metazoa and underlie epithelia as, a component of basement membranes. The networks are essential for tissue, function and are defective in disease. They are assembled by the, oligomerization of triple-helical protomers that are linked end-to-end. At, the C terminus, two protomers are linked head-to-head by interactions of, their trimeric noncollagenous domains, forming a hexamer structure. This, linkage in the alpha1.alpha2 network is stabilized by a putative covalent, Met-Lys cross-link between the trimer-trimer interface (Than, M. E., Henrich, S., Huber, R., Ries, A., Mann, K., Kuhn, K., Timpl, R., Bourenkov, G. P., Bartunik, H. D., and Bode, W. (2002) Proc. Natl. Acad., Sci. U. S. A. 99, 6607-6612) forming a nonreducible dimer that connects, the hexamer. In the present study, this cross-link was further, investigated by: (a) comparing the 1.5-A resolution crystal structures of, the alpha1.alpha2 hexamers from bovine placenta and lens capsule basement, membranes, (b) mass spectrometric analysis of monomer and nonreducible, dimer subunits of placenta basement membrane hexamers, and (c) hexamer, dissociation/re-association studies. The findings rule out the novel, Met-Lys cross-link, as well as other covalent cross-links, but establish, that the nonreducible dimer is an inherent structural feature of a, subpopulation of hexamers. The dimers reflect the reinforced, stabilization, by noncovalent forces, of the connection between two, adjoining protomers of a network. The reinforcement extends to other types, of collagen IV networks, and it underlies the cryptic nature of a B-cell, epitope of the alpha3.alpha4.alpha5 hexamer, implicating the stabilization, event in the etiology and pathogenesis of Goodpasture autoimmune disease.

About this Structure

1T61 is a Protein complex structure of sequences from Bos taurus with K, CL, CA and GOL as ligands. Full crystallographic information is available from OCA.

Reference

The alpha1.alpha2 network of collagen IV. Reinforced stabilization of the noncollagenous domain-1 by noncovalent forces and the absence of Met-Lys cross-links., Vanacore RM, Shanmugasundararaj S, Friedman DB, Bondar O, Hudson BG, Sundaramoorthy M, J Biol Chem. 2004 Oct 22;279(43):44723-30. Epub 2004 Aug 5. PMID:15299013

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